Electroporation conditions for linear DNA fragments

Eppendorf Electroporator 2510
Cuvette: 0.1 cm gap
1350V
600 Ohm
10µF
Time constant 4.5 – 5.0 ms
[Gene Bridges]

BioRad Gene Pulser II
Cuvette: 0.2 cm gap
2.5 kV
200 Ohm
25µF
Time constant 4.5 – 5.0 ms
[Chaveroche et al 2000]

BioRad Gene Pulser
Cuvette: 0.1 cm gap
1.8 kV
200 Ohm
25µF
Time constant 4.5 – 5.0 ms
[Wang et al 2005]

BioRad Pulse Controller
Cuvette: 0.1 cm gap
1.8kV
25µF
Low Range 200
High Range 500
[University of Wisconsin - Madison]


Remark
Eppendorf Operation Manual version 1.0 Electroporator 2510 page 11:
“Linear DNA has been shown to be about 104-fold less active than the some DNA transformed in circular form!”