Strain modifications

We have over 10 years of fee-for-service experience in strain and plasmid modifications, all very well made in Germany.

Escherichia coli is one important producer organism in industrial biotechnology and represents the best studied and understood prokaryotic model organism in microbiology. Genetic modified strains are used constantly to express several industrial important enzymes, fine chemicals and, most importantly, hormones such as somatostatin and insulin. In the last years metabolic engineering has become more and more attractive to optimize strains to meet commercial objectives by modifying metabolic pathways or even introducing entirely new ones.

Recombineering (Red/ET recombination) permits a defined and rapid access to the chromosomal modification of E. coli by (multiple) gene disruption, deletion and insertion, as well as gene modification by introducing (point) mutations in a seamless manner. Further, reporter genes or taqs can be integrated to investigate relevant metabolic pathways. Red/ET recombination also allows promotor fine tuning to optimize gene expression in order to remove bottlenecks or to reduce activities of metabolic pathways producing unwanted by-products.

Gene Bridges offers its know-how in micro- and molecular biology plus experience in White Biotechnology for the creation of any adjusted E. coli strain.

Costumers only name the relevant strain, the gene of interest and describe the desired modification while our service team takes care of the rest!

Our service includes:

  • customized construct design

  • decent timeline and regular progress updates

  • final strain confirmed by PCR and sequencing of modified region

  • if desired: documentation in GMP-like manner

We can offer a diverse spectrum of modifications:

  • (multiple) gene or fragment disruption, deletion and insertion

  • (multiple) seamless gene modification / point mutation

  • promotor fine tuning, replacement of chromosomal promotor

  • subcloning

  • plasmid modification

Please contact our service team in Heidelberg, Germany: contact@genebridges.com

Example E. coli modifications

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