E. coli strain GB05-dir

€620.00*

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Description

Full-length Rac prophage protein RecE and its partner RecT mediate highly efficient linear-to-linear homologous recombination mechanistically distinct from conventional recombineering mediated by Redα and Redβ from lambda phage. E. coli strain GB05-dir enables Direct Cloning (DC) and 4-Way Recombineering (4WR) with DNA fragments without the necessity to maintain an expression plasmid in the cells since the necessary genes are located on the chromosome.

Features

  • Direct Cloning (DC) of a linaer DNA fragment into a linear plasmid backbone

  • 4-Way Recombineering (4WR), a steamlined way to assemble novel plasmids in a single in vivo recombination step from up to four linear DNA fragments (Read the new 4WR Application Note)

  • E. coli strain harboring an arabinose inducible ETγA operon (full length recE, recT, redγ, and recA) at the ybcC locus

Applications

  • Cloning of PCR products

  • Cloning of linear DNA fragments from digested genomic DNA

  • Plasmid assembly from up to four linear DNA fragments, e.g. of Targeting Vectors for Recombineering And Cas9-assisted tagging (RAC-tagging)

Contents

  • Glycerol stock of Direct Cloning-proficient E. coli strain GB05-dir

  • Linear control plasmid backbone (ColE1 origin of replication and an ampicillin resistance marker gene)

  • Linear control insert (PCR product)

  • Protocol

Distributors worldwide

Asia

China Shanghai QF Biosciences
India

Biotech Desk

or

AGILITY BIOTECH

Biotech Desk

Israel BioTag
Japan Funakoshi Funakoshi
Korea Mbiotech
Singapore
Malaysia
Thailand
Vietnam
iDNA
Taiwan Gendiscovery

Europe

Germany
Austria
Switzerland
BioCat BioCat
France Biovalley
Spain Quimigen
The Netherlands Brunschwig Chemie
United Kingdom
Ireland
Cambio
Denmark
Norway
Sweden
Finland
Estonia
Immuno Diagnostic
Estonia
Latvia
Lithuania
Nanodiagnostika

North America

USA B-Bridge International B-Bridge International

South America

Chile Valquim

Troubleshooting

Should you have any problems using Red/ET technology, please consult our Troubleshooting Guide first.

Please complete all required fields marked with a asterix (*).

 
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How to contact our support

Should you have any further questions, please contact us directly.

Gene Bridges GmbH

Im Neuenheimer Feld 584

69120 Heidelberg

Germany

P +49 6221 13708 11

F +49 6221 13708 29

Technical inquiries:

contact@genebridges.com

Commercial licensing:

licenses@genebridges.com

* Prices may vary. Please check our list of distributors. Shipping charges are 45.00 EUR for Europe, 120.00 EUR for Overseas, unless you provide us with your forwarder's customer account number. Potential import duties and customs clearance costs will be charges directly to the customer.

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Shanghai QF Biosciences
Shanghai QF Biosciences
NeuProCell
Biotech Desk
NeuProCell
Biotech Desk
BioTag
BioTag
Funakoshi
Funakoshi
Mbiotech
Mbiotech
iDNA
iDNA
iDNA
iDNA
iDNA
iDNA
iDNA
iDNA
Gendiscovery
Gendiscovery
BioCat
BioCat
BioCat
BioCat
BioCat
BioCat
Biovalley
Biovalley
Quimigen
Quimigen
Brunschwig Chemie
Brunschwig Chemie
Cambio
Cambio
Cambio
Cambio
Immuno Diagnostic
Immuno Diagnostic
Immuno Diagnostic
Immuno Diagnostic
Immuno Diagnostic
Immuno Diagnostic
Immuno Diagnostic
Immuno Diagnostic
Immuno Diagnostic
Nanodiagnostika
Immuno Diagnostic
Nanodiagnostika
Nanodiagnostika
Nanodiagnostika
Nanodiagnostika
Nanodiagnostika
B-Bridge International
B-Bridge International
Valquim
Valquim