E. coli strain GB05-dir
€620.00*
Order now€620.00*
Order nowFull-length Rac prophage protein RecE and its partner RecT mediate highly efficient linear-to-linear homologous recombination mechanistically distinct from conventional recombineering mediated by Redα and Redβ from lambda phage. E. coli strain GB05-dir enables Direct Cloning (DC) and 4-Way Recombineering (4WR) with DNA fragments without the necessity to maintain an expression plasmid in the cells since the necessary genes are located on the chromosome.
Direct Cloning (DC) of a linaer DNA fragment into a linear plasmid backbone
4-Way Recombineering (4WR), a steamlined way to assemble novel plasmids in a single in vivo recombination step from up to four linear DNA fragments (Read the new 4WR Application Note)
E. coli strain harboring an arabinose inducible ETγA operon (full length recE, recT, redγ, and recA) at the ybcC locus
Cloning of PCR products
Cloning of linear DNA fragments from digested genomic DNA
Plasmid assembly from up to four linear DNA fragments, e.g. of Targeting Vectors for Recombineering And Cas9-assisted tagging (RAC-tagging)
Glycerol stock of Direct Cloning-proficient E. coli strain GB05-dir
Linear control plasmid backbone (ColE1 origin of replication and an ampicillin resistance marker gene)
Linear control insert (PCR product)
Protocol
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Should you have any problems using Red/ET technology, please consult our Troubleshooting Guide first.
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Gene Bridges GmbH
Im Neuenheimer Feld 584
69120 Heidelberg
Germany
P +49 6221 13708 11
F +49 6221 13708 29
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