E. coli strain GB05-dir
€620.00*
Order nowDescription
Full-length Rac prophage protein RecE and its partner RecT mediate highly efficient linear-to-linear homologous recombination mechanistically distinct from conventional recombineering mediated by Redα and Redβ from lambda phage. E. coli strain GB05-dir enables Direct Cloning (DC) and 4-Way Recombineering (4WR) with DNA fragments without the necessity to maintain an expression plasmid in the cells since the necessary genes are located on the chromosome.
Features
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Direct Cloning (DC) of a linaer DNA fragment into a linear plasmid backbone
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4-Way Recombineering (4WR), a steamlined way to assemble novel plasmids in a single in vivo recombination step from up to four linear DNA fragments (Read the new 4WR Application Note)
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E. coli strain harboring an arabinose inducible ETγA operon (full length recE, recT, redγ, and recA) at the ybcC locus
Applications
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Cloning of PCR products
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Cloning of linear DNA fragments from digested genomic DNA
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Plasmid assembly from up to four linear DNA fragments, e.g. of Targeting Vectors for Recombineering And Cas9-assisted tagging (RAC-tagging)
Contents
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Glycerol stock of Direct Cloning-proficient E. coli strain GB05-dir
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Linear control plasmid backbone (ColE1 origin of replication and an ampicillin resistance marker gene)
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Linear control insert (PCR product)
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Protocol
Distributors worldwide
Asia
| China | Shanghai QF Biosciences |  |
| India |
or |
|
| Israel | BioTag |  |
| Japan | Funakoshi |  |
| Korea | Mbiotech |  |
| Singapore Malaysia Thailand Vietnam |
iDNA |  |
| Taiwan | Gendiscovery |  |
Europe
| Germany Austria Switzerland |
BioCat |  |
| France | Biovalley |  |
| Spain | Quimigen |  |
| The Netherlands | Brunschwig Chemie |  |
| United Kingdom Ireland |
Cambio |  |
| Denmark Norway Sweden Finland Estonia |
Immuno Diagnostic |  |
| Estonia Latvia Lithuania |
Nanodiagnostika |  |
North America
| USA | AS ONE International, INC. |  |
South America
| Chile | Valquim |  |
Troubleshooting
Should you have any problems using Red/ET technology, please consult our Troubleshooting Guide first.
Please complete all required fields marked with a asterix (*).
How to contact our support
Should you have any further questions, please contact us directly.
Gene Bridges GmbH
Im Neuenheimer Feld 584
69120 Heidelberg
Germany
P +49 6221 13708 11
F +49 6221 13708 29
Technical inquiries:
Commercial licensing:
* Prices may vary. Please check our list of distributors. Shipping charges are 45.00 EUR for Europe, 120.00 EUR for Overseas, unless you provide us with your forwarder's customer account number. Potential import duties and customs clearance costs will be charges directly to the customer.
