Quick & Easy Conditional Knock Out Kit - FRT
€695.00*
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This kit is designed to integrate FRT sites in a vector plasmid at any intended position without the necessity to use restriction enzymes within 2-4 weeks
Applications
For fast and simple integration of FRT-sites into large vector plasmids at any intended position e.g. for generation of transgenic mouse models
Description
The included FRT-PGK-gb2-neo-FRT cassette is designed to allow neomycin / kanamycin selection in prokaryotic and eukaryotic cells. It combines a prokaryotic promoter (gb2) for expression of kanamycin resistance in E.coli with a eukaryotic promoter (PGK) for expression of neomycin resistance in mammalian cells. The included pCI-FLPe expression plasmid is coding for an improved FLP recombinase which shows a four to tenfold higher recombinational activity compared to the wildtype FLP enzyme at temperatures between 37°C (optimal growth temperature for E.coli) and 40°C (mouse body temperature). This kit can also be used to work on bacterial artificial chromosomes. High Red/ET efficiency plus convenient removal of the Red/ET recombination protein expression plasmid pRedET after recombination.
Contents
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Red/ET recombination protein expression plasmid pRed/ET. Any E. coli strain can be made Red/ET proficient by transformation with this plasmid
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FRT-PGK-gb2-neo-FRT template to be used for your own experiments
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Expression plasmid for enhanced FLP recombinase pCI-FLPe
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Positive controls to introduce a FRT site in a 18 kb high copy plasmid
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Detailed protocols, descriptions of plasmids, maps and sequences
Sequences
FRT-PGK-gb2-neo-FRT
FRT promoter neoR terminator
AATTAACCCTCACTAAAGGGCGGCCGCGAAGTTCCTATTCTCTAGAAAGTATAGG AACTTCATTCTACCGGGTAGGGGAGGCGCTTTTCCCAAGGCAGTCTGGAGCATGC GCTTTAGCAGCCCCGCTGGGCACTTGGCGCTACACAAGTGGCCTCTGGCCTCGCA CACATTCCACATCCACCGGTAGGCGCCAACCGGCTCCGTTCTTTGGTGGCCCCTT CGCGCCACCTTCCACTCCTCCCCTAGTCAGGAAGTTCCCCCCCGCCCCGCAGCTC GCGTCGTGCAGGACGTGACAAATGGAAGTAGCACGTCTCACTAGTCTCGTGCAGA TGGACAGCACCGCTGAGCAATGGAAGCGGGTAGGCCTTTGGGGCAGCGGCCAATA GCAGCTTTGCTCCTTCGCTTTCTGGGCTCAGAGGCTGGGAAGGGGTGGGTCCGGG GGCGGGCTCAGGGGCGGGCTCAGGGGCGGGGCGGGCGCCCGAAGGTCCTCCGGAG GCCCGGCATTCTGCACGCTTCAAAAGCGCACGTCTGCCGCGCTGTTCTCCTCTTC CTCATCTCCGGGCCTTTCGACCTGCAGCAGCACGTGTTGACAATTAATCATCGGC ATAGTATATCGGCATAGTATAATACGACAAGGTGAGGAACTAAACCATGGGATCG GCCATTGAACAAGATGGATTGCACGCAGGTTCTCCGGCCGCTTGGGTGGAGAGGC TATTCGGCTATGACTGGGCACAACAGACGATCGGCTGCTCTGATGCCGCCGTGTT CCGGCTGTCAGCGCAGGGGCGCCCGGTTCTTTTTGTCAAGACCGACCTGTCCGGT GCCCTGAATGAACTGCAGGACGAGGCAGCGCGGCTATCGTGGCTGGCCACGACGG GCGTTCCTTGCGCAGCTGTGCTCGACGTTGTCACTGAAGCGGGAAGGGACTGGCT GCTATTGGGCGAAGTGCCGGGGCAGGATCTCCTGTCATCTCACCTTGCTCCTGCC GAGAAAGTATCCATCATGGCTGATGCAATGCGGCGGCTGCATACGCTTGATCCGG CTACCTGCCCATTCGACCACCAAGCGAAACATCGCATCGAGCGAGCACGTACTCG GATGGAAGCCGGTCTTGTCGATCAGGATGATCTGGACGAAGAGCATCAGGGGCTC GCGCCAGCCGAACTGTTCGCCAGGCTCAAGGCGCGCATGCCCGACGGCGAGGATC TCGTCGTGACCCATGGCGATGCCTGCTTGCCGAATATCATGGTGGAAAATGGCCG CTTTTCTGGATTCATCGACTGTGGCCGGCTGGGTGTGGCGGACCGCTATCAGGAC ATAGCGTTGGCTACCCGTGATATTGCTGAAGAGCTTGGCGGCGAATGGGCTGACC GCTTCCTCGTGCTTTACGGTATCGCCGCTCCCGATTCGCAGCGCATCGCCTTCTA TCGCCTTCTTGACGAGTTCTTCTGAGCGGGACTCTGGGGTTCGAATAAAGACCGA CCAAGCGACGTCTGAGAGCTCCCTGGCGAATTCGGTACCAATAAAAGAGCTTTAT TTTCATGATCTGTGTGTTGGTTTTTGTGTGCGGCGCGGAAGTTCCTATTCTCTAG AAAGTATAGGAACTTCCTCGAGCCCTATAGTGAGTCGTATTAT
Distributors worldwide
Asia
| China | Shanghai QF Biosciences |  |
| India |
or |
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| Israel | BioTag |  |
| Japan | Funakoshi |  |
| Korea | Mbiotech |  |
| Singapore Malaysia Thailand Vietnam |
iDNA |  |
| Taiwan | Gendiscovery |  |
Europe
| Germany Austria Switzerland |
BioCat |  |
| France | Biovalley |  |
| Spain | Quimigen |  |
| The Netherlands | Brunschwig Chemie |  |
| United Kingdom Ireland |
Cambio |  |
| Denmark Norway Sweden Finland Estonia |
Immuno Diagnostic |  |
| Estonia Latvia Lithuania |
Nanodiagnostika |  |
North America
| USA | AS ONE International, INC. |  |
South America
| Chile | Valquim |  |
Troubleshooting
Should you have any problems using Red/ET technology, please consult our Troubleshooting Guide first.
Please complete all required fields marked with a asterix (*).
How to contact our support
Should you have any further questions, please contact us directly.
Gene Bridges GmbH
Im Neuenheimer Feld 584
69120 Heidelberg
Germany
P +49 6221 13708 11
F +49 6221 13708 29
Technical inquiries:
Commercial licensing:
* Prices may vary. Please check our list of distributors. Shipping charges are 45.00 EUR for Europe, 120.00 EUR for Overseas, unless you provide us with your forwarder's customer account number. Potential import duties and customs clearance costs will be charges directly to the customer.
